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rnhB [Wed Jan 20 2016 16:02:22 GMT+0100 (CET)]
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rnhB [Wed Jan 20 2016 16:02:22 GMT+0100 (CET)]

RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication
locus
BSU16060
pI
5.00
mw
28.00 kDa
function
endonucleolytic cleavage of RNA in
RNA-DNA hybrid molecules
product
Mn2 -dependent RNase HII
essential
no
synonyms
rnh
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]

    • [SW|Categories] containing this gene/protein

  • [SW|DNA replication], [SW|RNAses]

    • This gene is a member of the following [SW|regulons]

  • [SW|SigD regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,677,451 -> 1,678,218

    Phenotypes of a mutant

  • increased mutation rate [Pubmed|23882084]
  • a ''[protein|search|rnhB] [protein|search|rnhC]'' double mutant grows poorly [Pubmed|23882084]
  • a ''[protein|search|rnhB] [protein|search|mutS]-[protein|search|mutL]'' mutant is not viable [Pubmed|23882084]

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
  • removal of single rNMPs incorporated into DNA by DNA polymerase during [SW|DNA replication] [Pubmed|23882084]

    • Protein family

  • [SW|RNase] HII family (according to Swiss-Prot)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operon

  • [SW|rnhB]-[SW|ylqG]-[SW|ylqH] [Pubmed|26577401]

    • [SW|Sigma factor]

  • [SW|SigD] [Pubmed|26577401]

    • Biological materials

    Mutant

  • BP423 (''ΔrnhB::aphA3'') available in [SW|Fabian Commichau]'s lab

    • Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], based on [SW|pGP380], expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in [SW|Fabian Commichau]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

    • References

    Reviews

  • 19228197,24464998

    • Original publications

  • 9888800,10094689,12417313,17905985,23882084,26577401