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rnhB [2017-10-30 18:31:29]
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rnhB [2017-10-30 18:31:29]

RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication
Locus
BSU16060
Isoelectric point
5.52
Molecular weight
28.20 kDa
Protein length
255 aa Sequence Blast
Gene length
765 bp Sequence Blast
Function
endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules
Product
Mn2 -dependent RNase HII
Essential
no
E.C.
3.1.26.4
Synonyms
rnh
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA replication
  • Information processing → RNA synthesis and degradation → RNases → Endoribonucleases

    • Gene

    Coordinates
    1,677,451 → 1,678,218

    Phenotypes of a mutant

  • increased mutation rate PubMed
  • a rnhB rnhC double mutant grows poorly PubMed
  • a rnhB mutS-mutL mutant is not viable PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
  • removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication PubMed

    • Protein family

  • RNase HII family (according to Swiss-Prot)

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    rnhB-ylqG-ylqH
    Description
    PubMed

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon
    • view in new tab

    Genes
    rbgA-rnhB-ylqG-ylqH
    Description
    PubMed
    view in new tab

    Biological materials

    Mutant

  • BP423 (ΔrnhB::aphA3) available in Fabian Commichau's lab
  • BKE16060 (ΔrnhB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTCTCTTCTCCCTTAC, downstream forward: _UP4_TAAATCACCATGGACAAGGA
  • BKK16060 (ΔrnhB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTCTCTTCTCCCTTAC, downstream forward: _UP4_TAAATCACCATGGACAAGGA

    • Expression vector

  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, based on pGP380, expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

    • References

    Reviews

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    Original publications

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