SubtiBank SubtiBank
dnaJ
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dnaJ

heat-shock protein (activation of DnaK)
Locus
BSU_25460
Isoelectric point
7.92
Molecular weight
40.69 kDa
Protein length
375 aa Sequence Blast
Gene length
1128 bp Sequence Blast
Function
protein quality control
Product
heat-shock protein (activation of DnaK)
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,624,785 → 2,625,912

The protein

Protein family

  • dnaJ family (single member, according to UniProt)
  • Domains

  • J domain (aa 5-69) (according to UniProt)
  • Structure

  • 3LZ8 (from Klebsiella pneumoniae, 34% identity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • HrcA: repression, PubMed, in HrcA regulon
  • Regulation

  • induced by heat shock (HrcA) PubMed
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    Biological materials

    Mutant

  • BKE25460 (ΔdnaJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCGCTTCACTCTCCCG, downstream forward: _UP4_TAATTATTGGATGAGGAGTT
  • BKK25460 (ΔdnaJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCGCTTCACTCTCCCG, downstream forward: _UP4_TAATTATTGGATGAGGAGTT
  • Labs working on this gene/protein

  • Wolfgang Schumann, Bayreuth University, Germany Homepage
  • References

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