qcrA

Rieske factor, menaquinol:cytochrome c oxidoreductase (iron-sulfur subunit), component of the cytochrome bc1 complex

Locus: BSU_22560

Isoelectric point: 6.08

Molecular weight: 18.59 kDa

Protein length: 167 aa

Gene length: 504 bp

Function: respiration

Product: menaquinol:cytochrome c oxidoreductase (iron-sulfur subunit)

Essential: no

Synonyms: bfcA,petC

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Respiration → Respiration/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,364,589 → 2,365,092

Phenotypes of a mutant

• severe growth defect PubMed

The protein

Domains

• Rieske domain (aa 59-158) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 2ZT9 (from Nostoc sp., 31% identity) PubMed

Localization

• cell membrane PubMed
• delivered to the membrane by the TatAY-TatCY protein secretion system, this depends on prior dusulfide bond formation and co-factor insertion PubMed

Expression and Regulation

Operons

• Genes: qcrA-qcrB-qcrC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • induced by oxygen limitation (ResD) PubMed

Additional information

• highly expressed at high iron concentrations PubMed

Biological materials

Mutant

• BKE22560 (ΔqcrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCTTCTCCCCCCTCTA, downstream forward: _UP4_GCAAAGCCTAAGGGGGAAGG
• BKK22560 (ΔqcrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCTTCTCCCCCCTCTA, downstream forward: _UP4_GCAAAGCCTAAGGGGGAAGG

References

Reviews

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Original publications

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