dnmA

DNA methyltransferase

locus: BSU_06760

pI: 5.67

mw: 100.96 kDa

protein length: 879 aa

gene length: 2640 bp

function: genome-wide N6-methyladenosine modifications at non-palindromic recognition sites to control promoter activities

product: DNA methyltransferase

essential: no

synonyms: yeeA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.9|Other regulators]

Gene

Coordinates: 740,288 → 742,927

The protein

Catalyzed reaction/ biological activity

• formation of m6A DNA modifications at non-palindromic sites [pubmed|32324221]

Structure

• [PDB|5HR4] (from Methylophilus methylotrophus, 38% identity) [pubmed|27082731]

Expression and Regulation

Operons

• genes: [gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]-[gene|5B5014A68D777DB79179904D5FD3D14DEE0BF1F4|yefC]-[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]-[gene|2E55D086DD5023B98929FB8B61238C00E66B8BCB|yeeB]-[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A959 (yeeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/959 NBRP B. subtilis, Japan]
• BKE06760 (Δ[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCGTTTCCCCCATTAA, downstream forward: _UP4_ACAGAACGGTAGGTGTAAGG
• BKK06760 (Δ[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCGTTTCCCCCATTAA, downstream forward: _UP4_ACAGAACGGTAGGTGTAAGG

References

Research papers

• 27082731,32324221