polX [2017-8-11 16:0:3]

DNA polymerase X, involved in DNA repair, generates adaptive mutations by error-prone processing of A/P sites

locus: BSU28590

pI: 5.31

mw: 63.95 kDa

protein length: 570 aa

gene length: 1710 bp

function: DNA repair

product: DNA polymerase X

essential: no

ec: 2.7.7.7

synonyms: yshC

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 2,923,314 → 2,925,026

The protein

Catalyzed reaction/ biological activity

• template-dependent DNA polymerase, fills single nucleotide gaps [Pubmed|18938175]
• has intrinsic 3'-5' exonuclease activity for resecting unannealed 3'-termini in gapped DNA substrates [Pubmed|18776221]
• has intrinsic apurinic/apyrimidinic (AP) endonuclease activity [Pubmed|20974932]

Protein family

• DNA polymerase X family

Expression and Regulation

Operons

• genes: [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]-[gene|93726C423313C653C4F71CB107FA12F686959E41|yshB]-[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|mutSB]-[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]
  description: [Pubmed|22383849]

  regulation

  • constitutively expressed [Pubmed|23701187]

Biological materials

Mutant

• MGNA-B003 (yshC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1002 NBRP B. subtilis, Japan]

Labs working on this gene/protein

• [SW|Margarita Salas], Madrid, Spain [http://www.cbm.uam.es/mkfactory.esdomain/webs/CBMSO/plt_LineasInvestigacion.aspx?IdObjeto=28 link]

References

Reviews

• 22933559

Original publications

• 20974932,18938175,18776221,22844091,24914186,28254425