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degU [2019-08-28 12:30:26]
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degU [2019-08-28 12:30:26]

two-component response regulator, regulation of degradative enzyme expression, genetic competence, biofilm formation, capsule biosynthesis (together with SwrA), non-phosphorylated DegU is required for swarming motility
Locus
BSU_35490
Isoelectric point
5.60
Molecular weight
25.72 kDa
Protein length
229 aa Sequence Blast
Gene length
690 bp Sequence Blast
Function
regulation of degradative enzymes, genetic competence, and other adaptive responses
Product
two-component response regulator
Essential
no
Synonyms
sacU,iep

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,644,607 → 3,645,296

Phenotypes of a mutant

  • defect in biofilm formation PubMed, this can be suppressed by DegU-independent expression of BslA PubMed
  • the mutation suppresses the mucoid phenotype of motA or motB mutants due to reduced expression of the capB-capC-capA-capE operon PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • transcription activator and repressor (see DegU regulon)
  • DegU-P represses expression of the fla/che operon (flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB) in the absence of SwrAA/2, and switches to an activator of the operon in the presence of SwrAA/2 PubMed
  • Modification

  • phosphorylated by DegS on Asp-56 PubMed, this modulates DNA-binding activity
  • phosphorylation by DegS occurs in response to inhibition of flagellar rotation PubMed
  • Effectors of protein activity

  • DNA-binding activity of DegU is inhibited by RapG PubMed
  • DegU-P is degraded by ClpC-ClpP PubMed
  • Structure

  • 4GVP (VraR from Staphylococcus aureus, 35% identity) PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • DegU-P is degraded by ClpC-ClpP PubMed
  • accumulation of DegU-P results in decreased transcription of the epsA-epsO and tapA-sipW-tasA operons due to increased levels of Spo0A-P PubMed
  • information on binding sites can be found in the PRODORIC2 database
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • DegU: activation, in DegU regulon
  • TnrA: repression, PubMed, in TnrA regulon
  • CcpA: activation, PubMed, in CcpA regulon
  • SinR: repression, PubMed, in SinR regulon
  • Regulation

  • induced by glucose (CcpA) PubMed
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    Genes
    Description

    Regulation

  • induced by glucose (CcpA) PubMed
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    Biological materials

    Mutant

  • GP2644(degU::aphA3), available in Jörg Stülke's lab
  • BKE35490 (ΔdegU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT, downstream forward: _UP4_TAGTATAATAGGAGACTTGC
  • BKK35490 (ΔdegU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT, downstream forward: _UP4_TAGTATAATAGGAGACTTGC
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • References

    Reviews

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    The DegU regulon

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    Original Publications

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