yxiM

similar to rhamnogalacturonan acetylesterase

locus: BSU_39120

pI: 6.62

mw: 41.68 kDa

protein length: 382 aa

gene length: 1149 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 4,017,508 → 4,018,656

The protein

Protein family

• [SW|'GDSL' lipolytic enzyme family] (according to UniProt)

Paralogous protein(s)

• [protein|578B79EB64B804FCAACA73AB22458481371A815C|YesY], [protein|585CA3154C0405697C4707E5B1D9359963999C54|YesT]

Structure

• [PDB|2O14]

Expression and Regulation

Operons

• genes: [gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]-[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]
  description: [Pubmed|10746760]

  regulation

  • expression declines in the stationary phase [Pubmed|23175651]

Biological materials

Mutant

• MGNA-B719 (yxiM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1718 NBRP B. subtilis, Japan]
• GP1116 (spc), available in [SW|Stülke] lab
• BKE39120 (Δ[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT, downstream forward: _UP4_TAATACATCATTTTTCGACA
• BKK39120 (Δ[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT, downstream forward: _UP4_TAATACATCATTTTTCGACA