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hemC [2019-08-08 10:13:45]
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hemC [2019-08-08 10:13:45]

hydroxymethylbilane synthase
locus
BSU_28150
pI
5.09
mw
34.68 kDa
protein length
314 aa Sequence Blast
gene length
945 bp Sequence Blast
function
heme biosynthesis
product
hydroxymethylbilane synthase
essential
no
ec
2.5.1.61
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]

    • Gene

    Coordinates
    2,875,951 → 2,876,895

    The protein

    Catalyzed reaction/ biological activity

  • 4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3 (according to Swiss-Prot)

    • Protein family

  • HMBS family (single member, according to UniProt)

    • Structure

  • [PDB|4MLQ] (from ''Bacillus megaterium'', 70% identity) [Pubmed|24598743]

    • Expression and Regulation

    Operons

    count
    2
    genes
    [gene|B1413EB525000744BE8E429E760220A13BC4530D|hemA]-[gene|88616BA1E853E11BE0CB18F06287CA4C68143148|hemX]-[gene|D266E22E07B849E2AEB0EF232C49A2FE1EA4E02B|hemC]-[gene|33459D4389F85D60311FCE770BBA0ED9802BA342|hemD]-[gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]-[gene|A54B2C86638D474F5F892003B42C535B5B39A42E|hemL]
    description
    [Pubmed|1672867]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1672867], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]: repression, [Pubmed|11532148], in [regulon|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR regulon]

    • regulation

  • induced by hydrogen peroxide ([protein|search|PerR]) [Pubmed|11532148]
    • view in new tab

    Biological materials

    Mutant

  • BKE28150 (Δ[gene|D266E22E07B849E2AEB0EF232C49A2FE1EA4E02B|hemC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTCCTCCCGAATC, downstream forward: _UP4_CGTGTAAAACGGGAGCTTGA
  • BKK28150 (Δ[gene|D266E22E07B849E2AEB0EF232C49A2FE1EA4E02B|hemC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTCCTCCCGAATC, downstream forward: _UP4_CGTGTAAAACGGGAGCTTGA

    • References

    Reviews

  • 28123057

    • Original Publications

  • 10217486,1672867,11532148,24598743