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conE
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conE

ATPase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer
Locus
BSU_04940
Isoelectric point
5.51
Molecular weight
96.06 kDa
Protein length
797 aa Sequence Blast
Gene length
2394 bp Sequence Blast
Function
conjugative transfer of ICEBs1
Product
ATPase, part of the type IV secretion system for DNA transfer
Essential
no
Synonyms
yddE

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
538,836 → 541,229

Phenotypes of a mutant

  • Defective for conjugal transfer of ICE''Bs1
  • The protein

    Catalyzed reaction/ biological activity

  • required for conjugative transfer of ICEBs1, putative ATPase PubMed
  • Protein family

  • HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4
  • Localization

  • peripheral membrane protein (via ConB), located at the cell poles PubMed
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • ImmR: repression, PubMed, in ImmR regulon
  • Regulation

  • strongly induced in the presence of salt (1.2 M NaCl) PubMed
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    Biological materials

    Mutant

  • BKE04940 (ΔconE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTTTTATACGATTCAACTT, downstream forward: _UP4_TTGTAAAAAGGGGTTGAATC
  • BKK04940 (ΔconE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTTTTATACGATTCAACTT, downstream forward: _UP4_TTGTAAAAAGGGGTTGAATC
  • Labs working on this gene/protein

  • Melanie Barker Berkmen, Suffolk University, Boston, MA Homepage
  • References

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