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cysK [2017-05-17 11:12:48]
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cysK [2017-05-17 11:12:48]

trigger enzyme: cysteine synthetase A and control of CymR activity
locus
BSU00730
pI
5.49
mw
32.67 kDa
protein length
308 aa Sequence Blast
gene length
924 bp Sequence Blast
function
biosynthesis of cysteine, control of CymR activity
product
trigger enzyme: cysteine synthetase A
essential
no
ec
2.5.1.47
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.2|Trigger enzymes that control gene expression by protein-protein interaction with transcription factors]

    • Gene

    Coordinates
    81,771 → 82,697

    Phenotypes of a mutant

  • constitutive expression of the ''[gene|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH]-[gene|58A5A7F12664EC38AC21E24C1AD89671D7E648CF|cysP]-[gene|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|sat]-[gene|3C71659E4868744A9301C26608EABF7B98217DCF|cysC]-[gene|92831D5A8E07A18BA6C6978123769D00D685BB45|ylnD]-[gene|9349C4646A56F933041EBE750F1BE86BAE7A60D9|sirB]-[gene|8BAB9B12D9D58A45446DC6CB7F48869089800FD3|ylnF]'' operon, auxotrophic for cysteine

    • The protein

    Catalyzed reaction/ biological activity

  • O(3)-acetyl-L-serine H2S = L-cysteine acetate (according to Swiss-Prot)

    • Protein family

  • cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)

    • Structure

  • [PDB|1Y7L] (from ''Haemophilus influenzae'', 39% identity, 52% similarity) [Pubmed|15838047]

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]-[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]-[gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]-[gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

    • regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|30480837], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • induced by heat stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30480837]
    • view in new tab

    genes
    [gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK]
    description
    [Pubmed|9084182]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11445163], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|12642660,19575568], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • repressed by casamino acids [Pubmed|12107147]
    • view in new tab

    Biological materials

    Mutant

  • 1A834 ( ''cysK''::''kan''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A834&Search=1A834 BGSC]
  • 1A801 ( ''cysK''::''spec''), [Pubmed|11445163], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A801&Search=1A801 BGSC]
  • 1A803 ( ''cysK''::''spec''), [Pubmed|11445163], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A803&Search=1A803 BGSC]
  • 1A946 ( ''cysK''::''spec''), [Pubmed|17056751], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A946&Search=1A946 BGSC]

    • Labs working on this gene/protein

  • [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

    • References

  • 16513748,17056751,18974048,16267287,12642660,11445163,12107147,15838047,9084182,15378759