cysK [Thu Jan 14 2016 18:13:16 GMT+0100 (CET)]

trigger enzyme: cysteine synthetase A and control of CymR activity

Locus: BSU00730

Isoelectric point: 5.00

Molecular weight: 32.00 kDa

Function: biosynthesis of cysteine, control of CymR activity

Product: trigger enzyme: cysteine synthetase A

Essential: no

E.C.: 2.5.1.47

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
• Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzymes that control gene expression by protein-protein interaction with transcription factors

Categories containing this gene/protein

• biosynthesis/ acquisition of amino acids, transcription factors and their control, trigger enzyme, most abundant proteins

This gene is a member of the following regulons

• CymR regulon, Spx regulon

Gene

Coordinates on the chromosome (coding sequence): 81,771 -> 82,697

Phenotypes of a mutant

• constitutive expression of the cysH-cysP-sat-cysC-ylnD-ylnE-ylnF operon, auxotrophic for cysteine

The protein

Catalyzed reaction/ biological activity

• O(3)-acetyl-L-serine H₂S = L-cysteine acetate (according to Swiss-Prot)

Protein family

• cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)

Structure

• 1Y7L (from Haemophilus influenzae, 39% identity, 52% similarity) PubMed

Interactions

• CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed

Expression and Regulation

Operon

• cysK PubMed

Sigma factor

• SigA PubMed

Regulation

• repressed by casamino acids PubMed
• repressed in the presence of cysteine (CymR) PubMed

Regulatory mechanism

• CymR: transcription repression PubMed
• Spx: transcription activation PubMed

Additional information

• belongs to the 100 [[most abundant proteins]] PubMed

Biological materials

Mutant

• 1A834 ( cysK::kan), [Pubmed| ], available at BGSC
• 1A801 ( cysK::spec), PubMed, available at BGSC
• 1A803 ( cysK::spec), PubMed, available at BGSC
• 1A946 ( cysK::spec), PubMed, available at BGSC

Labs working on this gene/protein

• [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

References

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