SubtiBank SubtiBank
cysK [Thu Jan 14 2016 18:13:16 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

cysK [Thu Jan 14 2016 18:13:16 GMT+0100 (CET)]

trigger enzyme: cysteine synthetase A and control of CymR activity
locus
BSU00730
pI
5.00
mw
32.00 kDa
function
biosynthesis of cysteine, control of CymR activity
product
trigger enzyme: cysteine synthetase A
essential
no
ec
2.5.1.47
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.2|Trigger enzymes that control gene expression by protein-protein interaction with transcription factors]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|transcription factors and their control], [SW|trigger enzyme], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CymR regulon], [SW|Spx regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    81,771 -> 82,697

    Phenotypes of a mutant

  • constitutive expression of the ''[protein|search|cysH]-[protein|search|cysP]-[protein|search|sat]-[protein|search|cysC]-[protein|search|ylnD]-[protein|search|ylnE]-[protein|search|ylnF]'' operon, auxotrophic for cysteine

    • The protein

    Catalyzed reaction/ biological activity

  • O(3)-acetyl-L-serine H2S = L-cysteine acetate (according to Swiss-Prot)

    • Protein family

  • cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)

    • Structure

  • [PDB|1Y7L] (from ''Haemophilus influenzae'', 39% identity, 52% similarity) [Pubmed|15838047]

    • [SW|Interactions]

  • [protein|search|CymR]-[protein|search|CysK], the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis [Pubmed|18974048]

    • Expression and Regulation

    Operon

  • ''[protein|search|cysK]'' [Pubmed|9084182]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11445163]

    • Regulation

  • repressed by casamino acids [Pubmed|12107147]
  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]

    • Regulatory mechanism

  • [protein|search|CymR]: transcription repression [Pubmed|16513748]
  • [protein|search|Spx]: transcription activation [Pubmed|12642660,19575568]

    • Additional information

  • belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

    • Biological materials

    Mutant

  • 1A834 ( ''cysK''::''kan''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A834&Search=1A834 BGSC]
  • 1A801 ( ''cysK''::''spec''), [Pubmed|11445163], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A801&Search=1A801 BGSC]
  • 1A803 ( ''cysK''::''spec''), [Pubmed|11445163], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A803&Search=1A803 BGSC]
  • 1A946 ( ''cysK''::''spec''), [Pubmed|17056751], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A946&Search=1A946 BGSC]

    • Labs working on this gene/protein

  • [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

    • References

  • 16513748,17056751,18974048,16267287,12642660,11445163,12107147,15838047,9084182,15378759