gerKB

nutrient receptor

locus: BSU_03720

pI: 8.92

mw: 41.61 kDa

protein length: 373 aa

gene length: 1122 bp

function: germination

product: nutrient receptor

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.4|APC superfamily]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.1|Germinant receptors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 422,982 → 424,103

The protein

Protein family

• [SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
• [SW|Spore germination protein (SGP) (TC 2.A.3.9) family] (according to UniProt)

Paralogous protein(s)

• [protein|D70EC7C02BC79BED724489ABCB6356447F215B87|GerBB], [protein|5FF739B8C087207039BB6DC293EE77F59337ED0B|GerAB], [protein|B14179D0EF5D3493E5CDB8E15378A5088B4FFD10|YndE]

[SW|Localization]

• spore inner membrane [Pubmed|21696470]

Expression and Regulation

Operons

• genes: [gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[gene|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC]-[gene|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]
  description: [pubmed|22383849]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16707705], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: repression, [Pubmed|16707705], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]

  regulation

  • expressed during sporulation ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG], [SW|SpoVT]) [Pubmed|16707705]

  additional information

  • 700 molecules are present per spore [PubMed|23749970]

Biological materials

Mutant

• BKE03720 (Δ[gene|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCACTTCCCCTTTC, downstream forward: _UP4_TGATAAAAAGAGCGGGGGGA
• BKK03720 (Δ[gene|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCACTTCCCCTTTC, downstream forward: _UP4_TGATAAAAAGAGCGGGGGGA

References

• 16707705,21696470,16352818,23396907,26731423,32210351