general stress protein, similar to glutamate synthase (ferredoxin), required for protection against paraquat stress
function
protection against paraquat stress
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]Gene
Coordinates
716,780 → 718,357
The protein
Paralogous protein(s)
[protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|GltA] [SW|Cofactors]
FMN or FAD [Pubmed|21635694][SW|Localization]
cell membrane (according to UniProt)Expression and Regulation
Operons
genes
[gene|D1AF9DA2594D2E2A94DC207A870C8495954A7A17|yerD]
description
[Pubmed|22383849]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528]view in new tabBiological materials
Mutant
MGNA-A925 (yerD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/925 NBRP B. subtilis, Japan]GP1184 (tet) available in [SW|Jörg Stülke]'s labBKE06590 (Δ[gene|D1AF9DA2594D2E2A94DC207A870C8495954A7A17|yerD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTCAGCCTCCCTTTT, downstream forward: _UP4_TAAAGAAACCGCCCTTGCCABKK06590 (Δ[gene|D1AF9DA2594D2E2A94DC207A870C8495954A7A17|yerD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTCAGCCTCCCTTTT, downstream forward: _UP4_TAAAGAAACCGCCCTTGCCAExpression vectors
pBP32 (expression vector for ''E. coli'' BL21; N-terminal Strep-tag-YerD fusion protein, based on [SW|pGP172]), available in [SW|Fabian Commichau]'s labFLAG-tag construct
GP1187 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lablabs
[SW|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]References
15805528,22383849,23033921,22582280