smpB

tmRNA-binding protein

locus: BSU_33600

pI: 10.42

mw: 17.95 kDa

protein length: 156 aa

gene length: 471 bp

function: trans-translation

product: tmRNA-binding protein

essential: no

synonyms: yvaI

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.12|Translation/ other]

Gene

Coordinates: 3,451,248 → 3,451,718

Phenotypes of a mutant

• impaired growth at high and low temperatures [Pubmed|15805528]
• inactivation of ''[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]'' reduces sporulation efficiency to 23% that of wild type cells; delayed entry into sporulation [Pubmed|26735940]
• essential in the absence of [protein|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|BrfA] [https://www.biorxiv.org/content/biorxiv/early/2019/08/15/732420.full.pdf reference]

The protein

Protein family

• smpB family (single member, according to UniProt)

Structure

• [PDB|1P6V] (from ''Aquifex aeolicus'', 55% identity, complex with the tRNA domain of [protein|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|SsrA]) [Pubmed|12904796]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|secG]-[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]-[gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]-[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
  description: [Pubmed|17369301]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • ''[protein|search|yvaK]'': induced by stress ([protein|search|SigB]) [Pubmed|11544224]

• genes: [gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
  description: [Pubmed|17369301]

  regulation

  • ''[protein|search|yvaK]'': [protein|search|SigB] [Pubmed|11544224]

• genes: [gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]-[gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]-[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
  description: [Pubmed|17369301]

  regulation

  • ''[protein|search|yvaK]'': induced by stress ([protein|search|SigB]) [Pubmed|11544224]

Biological materials

Mutant

• MGNA-A494 (yvaI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/494 NBRP B. subtilis, Japan]
• BKE33600 (Δ[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCAGAACCTCCTCTCT, downstream forward: _UP4_TAAAGGCATAGTGCTTGATT
• BKK33600 (Δ[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCAGAACCTCCTCTCT, downstream forward: _UP4_TAAAGGCATAGTGCTTGATT

labs

• [SW|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]

References

Reviews

• 12730326,10881189

Original publications

• 18977770,11395451,17369301,10881189,15805528,12904796,26735940