prpE [2019-07-31 09:42:43]

protein tyrosine phosphatase

locus: BSU_11630

pI: 6.75

mw: 27.31 kDa

protein length: 244 aa

gene length: 735 bp

function: unknown

product: protein tyrosine phosphatase

essential: no

ec: 3.6.1.17

synonyms: yjbP

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]

Gene

Coordinates: 1,239,387 → 1,240,121

Phenotypes of a mutant

• increased amounts of small aci-soluble spore proteins (SASPs) [Pubmed|21092197]

The protein

Catalyzed reaction/ biological activity

• H₂O + P¹,P⁴-bis(5'-guanosyl) tetraphosphate --> GMP + GTP + 2 H⁺ (according to Swiss-Prot)

Protein family

• prpE family (single member, according to UniProt)

Structure

• [PDB|4J6O] (PnkP from Thermocellum sp., 48% identity) [pubmed|23595150]

[SW|Localization]

• forespore (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|D1661093571DA4DEC5FBD8EDD3910879DAFDEE23|prpE]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B162 (yjbP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1161 NBRP B. subtilis, Japan]
• BKE11630 (Δ[gene|D1661093571DA4DEC5FBD8EDD3910879DAFDEE23|prpE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGGTCTCCTCCTTACC, downstream forward: _UP4_CGCCCCATATAAAAAAGGAG
• BKK11630 (Δ[gene|D1661093571DA4DEC5FBD8EDD3910879DAFDEE23|prpE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGGTCTCCTCCTTACC, downstream forward: _UP4_CGCCCCATATAAAAAAGGAG

References

• 12059787,16740944,21092197,22383849,23824080,23595150