ppsC

plipastatin synthetase

Locus: BSU_18320

Isoelectric point: 5.35

Molecular weight: 287.19 kDa

Protein length: 2555 aa

Gene length: 7668 bp

Function: production of the antibacterial compound plipastatin

Product: plipastatin synthetase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds

Gene

Coordinates: 1,974,881 → 1,982,548

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Paralogous protein(s)

• partial match (more than 1,000 amino acids): DhbF, PksJ, PksN, PpsA, PpsB, PpsE, SrfAC

Domains

• 2 Carrier domains (aa 967-1042, aa 2003-2077) (according to UniProt)

Structure

• 2VSQ (termination module of SrfAC, 37% identity) PubMed

Expression and Regulation

Operons

• Genes: ppsA-ppsB-ppsC-ppsD-ppsE
  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon

Biological materials

Mutant

• BKE18320 (ΔppsC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGCGGCATCAGTGAATCTC, downstream forward: _UP4_TAGAAAATCGTGACAGGAGA
• BKK18320 (ΔppsC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGCGGCATCAGTGAATCTC, downstream forward: _UP4_TAGAAAATCGTGACAGGAGA

References

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