ycnJ [2019-06-25 11:22:42]

copper transporter

locus: BSU03950

pI: 10.10

mw: 59.64 kDa

protein length: 541 aa

gene length: 1626 bp

function: uptake of copper

product: copper transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.1|Copper]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 446,801 → 448,426

Phenotypes of a mutant

• growth-defective under copper-limiting conditions [Pubmed|19168619]

The protein

Catalyzed reaction/ biological activity

• uptake of copper [Pubmed|19168619]

[SW|Localization]

• cell membrane [Pubmed|19168619]

Expression and Regulation

Operons

• genes: [gene|98D807FBB847BF731B5C236557C8F70347279CE0|ycnK]-[gene|D0F553E661A35C69380ACB01D9A96FC6C5088470|ycnJ]-[gene|1B7F59F954A1F20F75F5D4953F84CE58171741DE|ycnI]
  description: [Pubmed|22904286,22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22904286], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|98D807FBB847BF731B5C236557C8F70347279CE0|YcnK]: repression, [Pubmed|22904286,19168619], in [regulon|98D807FBB847BF731B5C236557C8F70347279CE0|YcnK regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|15101989], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by copper limitation ([protein|search|YcnK]) [Pubmed|22904286,19168619]

Biological materials

Mutant

• BKE03950 (Δ[gene|D0F553E661A35C69380ACB01D9A96FC6C5088470|ycnJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTCAGACACCACCTT, downstream forward: _UP4_AAGACCAATTAAGGAGTGTT
• BKK03950 (Δ[gene|D0F553E661A35C69380ACB01D9A96FC6C5088470|ycnJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTCAGACACCACCTT, downstream forward: _UP4_AAGACCAATTAAGGAGTGTT

labs

• [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

• 19168619,22904286,22383849