ahpC [2018-05-09 09:51:02]

alkyl hydroperoxide reductase (small subunit)

Locus: BSU40090

Isoelectric point: 4.28

Molecular weight: 20.48 kDa

Protein length: 187 aa

Gene length: 561 bp

Function: resistance against peroxide stres

Product: alkyl hydroperoxide reductase (small subunit)

Essential: no

E.C.: 1.11.1.15

Synonyms: perR

Genomic Context

Loading

Categories containing this gene/protein

• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
• Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

Gene

Coordinates: 4,118,950 → 4,119,513

The protein

Catalyzed reaction/ biological activity

• 2 R'-SH + ROOH = R'-S-S-R' + H₂O + ROH (according to Swiss-Prot)

Protein family

• ahpC/TSA family (according to Swiss-Prot)

Paralogous protein(s)

• AhpA

Paralogous protein

• AhpA

Modification

• phosphorylated on ser/ thr/ tyr PubMed

Structure

• 1WE0 (from Amphibacillus xylanus, 78% identity, 88% similarity) PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ahpC-ahpF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PerR: repression, PubMed, in PerR regulon

  Regulation

  • induced by H2O2 (PerR) PubMed

Biological materials

Mutant

• BKE40090 (ΔahpC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATGTATATTCCTCCTA, downstream forward: _UP4_GGTAAAATCTAAGGAGTGCA
• BKK40090 (ΔahpC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATGTATATTCCTCCTA, downstream forward: _UP4_GGTAAAATCTAAGGAGTGCA

References

Loading