yhjG [2019-06-27 12:25:40]

similar to monooxygenase

locus: BSU10500

pI: 6.69

mw: 54.19 kDa

protein length: 499 aa

gene length: 1500 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,122,862 → 1,124,361

The protein

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|4K2X] (from ''Streptomyces Rimosus'', 43% identity) [pubmed|23621493]

Expression and Regulation

Operons

• genes: [gene|D07BD51E1DDEDA3CE292570AAEAE14C27C064A67|yhjG]-[gene|F5A59CE7727E0851652BB4C0144009154AB4978B|yhjH]
  description: [Pubmed|22383849]

additional information

• [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• MGNA-B285 (yhjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1284 NBRP B. subtilis, Japan]
• BKE10500 (Δ[gene|D07BD51E1DDEDA3CE292570AAEAE14C27C064A67|yhjG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCACCACTCCTTTTGTT, downstream forward: _UP4_TAATTCAATTCGATTGTTTC
• BKK10500 (Δ[gene|D07BD51E1DDEDA3CE292570AAEAE14C27C064A67|yhjG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCACCACTCCTTTTGTT, downstream forward: _UP4_TAATTCAATTCGATTGTTTC

References

Research papers

• 23621493