yddT [2018-02-03T02:06:40.000Z]

unknown

locus: BSU05100

pI: 9.55

mw: 25.31 kDa

protein length: 228 aa

gene length: 684 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 556,762 → 557,449

Protein

Paralogous protein(s)

• [protein|25ACC4472A56ABF9F0FCA75CC24579FBC59E4C2F|YomL] (the two proteins differ in only 4 out of 228 amino acids)

Expression and Regulation

Operons

• genes: [gene|D04CF5261C840E9A1BC7FFE5B6F492A46EC0971C|yddT]
  description: [Pubmed|20817675]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• BKE05100 (Δ[gene|D04CF5261C840E9A1BC7FFE5B6F492A46EC0971C|yddT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAAATCCTCCTTGTG, downstream forward: _UP4_TAATTTTAAAATCACTTTGT
• BKK05100 (Δ[gene|D04CF5261C840E9A1BC7FFE5B6F492A46EC0971C|yddT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAAATCCTCCTTGTG, downstream forward: _UP4_TAATTTTAAAATCACTTTGT

Biological material

Mutant

• MGNA-C164 (yddT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2162 NBRP B. subtilis, Japan]
• BP116 (yddT::spc), available in[SW|Fabian Commichau]'s lab [Pubmed|24178028]

Expression vector

• IPTG inducible expression of Strep-yddT in E. coli: [SW|pGP2581] (in pGP172), available in [SW|Jörg Stülke]'s lab

References

• 17114254,23420519,20817675,24178028