yhdI [2019-08-28 08:59:51]

similar to transcriptional regulator ([SW|MocR/ GabR family])

locus: BSU_09480

pI: 6.80

mw: 52.60 kDa

protein length: 469 aa

gene length: 1410 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]

Gene

Coordinates: 1,026,255 → 1,027,664

The protein

Protein family

• [SW|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
• [SW|MocR/ GabR family ][Pubmed|22020104]

[SW|Domains]

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.

[SW|Cofactors]

• PLP

Structure

• [PDB|4MGR] ([protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|GabR], 40% identity) [pubmed|24127574]

Expression and Regulation

Operons

• genes: [gene|D0498CC264FFC4B4C0DECAEBBBB884DB1122A4B2|yhdI]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B480 (yhdI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1479 NBRP B. subtilis, Japan]
• BKE09480 (Δ[gene|D0498CC264FFC4B4C0DECAEBBBB884DB1122A4B2|yhdI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCATGTCTCCTTTGTA, downstream forward: _UP4_TAAATAAAAAACAGGCCCCG
• BKK09480 (Δ[gene|D0498CC264FFC4B4C0DECAEBBBB884DB1122A4B2|yhdI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCATGTCTCCTTTGTA, downstream forward: _UP4_TAAATAAAAAACAGGCCCCG

References

• 11756427,22020104,24127574