ptpZ [2019-02-27 17:31:10]

phosphotyrosine protein phosphatase, antagonist to PtkA

locus: BSU36240

pI: 6.66

mw: 28.81 kDa

protein length: 254 aa

gene length: 765 bp

function: protein tyrosine dephosphorylation

product: phosphotyrosine protein phosphatase, antagonist to PtkA

essential: no

ec: 3.1.3.48

synonyms: ywqE

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]

Gene

Coordinates: 3,731,005 → 3,731,769

The protein

Catalyzed reaction/ biological activity

• Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate (according to Swiss-Prot), dephosphorylation of [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|Ugd], [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|TuaD] and [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA] [Pubmed|15866923]

Protein family

• cpsB/capC family (according to Swiss-Prot)

Structure

• [PDB|3QY7] [Pubmed|21605684]

Expression and Regulation

Operons

• genes: [gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]-[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]-[gene|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd]
  description: [Pubmed|20815827,12970183]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20815827], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|26283769], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|26283769], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• MGNA-A077 (ywqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/77 NBRP B. subtilis, Japan]
• GP1609 (spc), available in [SW| Jörg Stülke]'s lab
• GP1610 (''[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]'', spc), available in [SW| Jörg Stülke]'s lab
• BKE36240 (Δ[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTAGCCCCCTTTTT, downstream forward: _UP4_TAACAGCCGATTCTCATTTC
• BKK36240 (Δ[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTAGCCCCCTTTTT, downstream forward: _UP4_TAACAGCCGATTCTCATTTC

References

• 15866923,12970183,21605684,20815827,21605684,20817675,26283769,27148221