ptpZ

phosphotyrosine protein phosphatase, antagonist to PtkA

Locus: BSU_36240

Isoelectric point: 6.66

Molecular weight: 28.81 kDa

Protein length: 254 aa

Gene length: 765 bp

Function: protein tyrosine dephosphorylation

Product: phosphotyrosine protein phosphatase, antagonist to PtkA

Essential: no

E.C.: 3.1.3.48

Synonyms: ywqE

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein phosphatases
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation

Gene

Coordinates: 3,731,005 → 3,731,769

The protein

Catalyzed reaction/ biological activity

• H₂O + O-phospho-L-tyrosyl-[protein] --> L-tyrosyl-[protein] + phosphate (according to UniProt)
• dephosphorylation of Ugd, TuaD and PtkA PubMed

Protein family

• Metallo-dependent hydrolases superfamily (according to UniProt)

Structure

• 3QY7 PubMed

Expression and Regulation

Operons

• Genes: tkmA-ptkA-ptpZ-ugd
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • AbrB: repression, PubMed, in AbrB regulon

Biological materials

Mutant

• MGNA-A077 (ywqE::erm), available at the NBRP B. subtilis, Japan
• GP1609 (spc), available in Jörg Stülke's lab
• GP1610 (ptkA-ptpZ, spc), available in Jörg Stülke's lab
• BKE36240 (ΔptpZ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCCTAGCCCCCTTTTT, downstream forward: _UP4_TAACAGCCGATTCTCATTTC
• BKK36240 (ΔptpZ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCCTAGCCCCCTTTTT, downstream forward: _UP4_TAACAGCCGATTCTCATTTC

References

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