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sda [Mon Aug 31 2015 13:55:42 GMT+0200 (CEST)]
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sda [Mon Aug 31 2015 13:55:42 GMT+0200 (CEST)]

locus
BSU25690
pI
5.61
mw
6.00 kDa
function
mediates a developmental checkpoint coupling
initiation of sporulation (phosphorylation of Spo0A)
to the function of replication initiation proteins
product
developmental checkpoint protein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases]

    • [SW|Categories] containing this gene/protein

  • [SW|phosphorelay]

    • This gene is a member of the following [SW|regulons]

  • [SW|DnaA regulon], [SW|LexA regulon]

    • Gene

    Phenotypes of a mutant

  • sporulates at a higher frequency
  • will sporulate in the presence of replication stress

    • The protein

    Catalyzed reaction/ biological activity

  • Sda is a checkpoint protein that is upregulated in response to replication stress [Pubmed|11207367]. Sda inhibits the autokinase activity of [protein|search|KinA] (and likely [protein|search|KinB] as well). This in its turn results in reduced levels of [Spo0A|search|Spo0A~P]. Thus, replication stressed cells will not initiate sporulation.

    • Structure

  • [PDB|3D36] (from ''Geobacillus stearothermophilus'', complex with [protein|search|KinB]) [Pubmed|19101565], [PDB|1PV0]

    • [SW|Interactions]

  • [protein|search|KinB]-[protein|search|Sda] [Pubmed|19465772]
  • [protein|search|KinA]-[protein|search|Sda] [Pubmed|15023339]

    • Additional information

  • [protein|search|Sda] is rapidly degraded by the [ClpP|search|ClpP/X] system because of some uncharged residues at its C-terminus [Pubmed|16796683]

    • Expression and Regulation

    Operon

  • ''[protein|search|sda]'' [Pubmed|11207367]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11207367]

    • Regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]

    • Regulatory mechanism

  • [protein|search|DnaA]: transcription activation under replication stress [Pubmed|17932079]
  • [protein|search|LexA]: transcription repression [Pubmed|16267290]

    • Biological materials

    Mutant

  • 1A1034 (no resistance), [Pubmed|16796683], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1034&Search=1A1034 BGSC]

    • Labs working on this gene/protein

  • [SW|Bill Burkholder] [http://burkholder.stanford.edu/ Homepage]

    • References

    Reviews

  • 22933559

    • Original publications

  • 11207367,16267290,19465772,15023339,16796683,19684115,17932079,17350039,19101565,20525796,26020636