sda [2020-09-10 09:30:10]

developmental checkpoint protein, controls the phosphorylation status of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]

locus: BSU_25690

pI: 5.61

mw: 6.02 kDa

protein length: 52 aa

gene length: 159 bp

function: mediates a developmental checkpoint coupling initiation of [category|SW 4.2|Sporulation] (phosphorylation of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A])to the function of replication initiation proteins

product: developmental checkpoint protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases]

Gene

Coordinates: 2,647,456 → 2,647,614

Phenotypes of a mutant

• sporulates at a higher frequency
• will sporulate in the presence of replication stress

The protein

Catalyzed reaction/ biological activity

• Sda is a checkpoint protein that is upregulated in response to replication stress [Pubmed|11207367]. Sda inhibits the autokinase activity of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA] (and likely [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB] as well). This in its turn results in reduced levels of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A~P]]]. Thus, replication stressed cells will not initiate sporulation.

Structure

• [PDB|3D36] (from ''Geobacillus stearothermophilus'', complex with [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB]) [Pubmed|19101565], [PDB|1PV0]

Additional information

• [protein|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|Sda] is rapidly degraded by the [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP/X]]] system because of some uncharged residues at its C-terminus [Pubmed|16796683]

Expression and Regulation

Operons

• genes: [gene|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda]
  description: [Pubmed|11207367]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11207367], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: activation, under replication stress [Pubmed|17932079], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]
  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]

  additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|sda]' [PubMed|20525796]

Biological materials

Mutant

• 1A1034 (no resistance), [Pubmed|16796683], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1034&Search=1A1034 BGSC]
• BKE25690 (Δ[gene|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGGGAGAGGCACCTCCTT, downstream forward: _UP4_TAATAGGAATTTGTCTATTT
• BKK25690 (Δ[gene|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGGGAGAGGCACCTCCTT, downstream forward: _UP4_TAATAGGAATTTGTCTATTT

labs

• [SW|Bill Burkholder] [http://burkholder.stanford.edu/ Homepage]

References

Reviews

• 22933559

Original publications

• 11207367,16267290,19465772,15023339,16796683,19684115,17932079,17350039,19101565,20525796,26020636,28752667