safA [2019-07-12 16:58:12]

morphogenetic protein associated with SpoVID, major organizer of the inner spore coat

Locus: BSU_27840

Isoelectric point: 5.75

Molecular weight: 43.07 kDa

Protein length: 387 aa

Gene length: 1164 bp

Function: spore coat formation

Product: morphogenetic protein

Essential: no

Synonyms: yrbA

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Not yet assigned

Gene

Coordinates: 2,844,675 → 2,845,838

Phenotypes of a mutant

• mis-assembly of the inner spore coat PubMed

The protein

Domains

• contains a N-acetylglucosamine-polymer-binding LysM domain at the N-terminus PubMed

Localization

• inner spore coat PubMed
• cortex-coat interface PubMed

Additional information

• Tgl cross-links SafA to other spore coat proteins PubMed

Expression and Regulation

Operons

• Genes: safA-coxA
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

Biological materials

Mutant

• MGNA-A011 (yrbA::erm), available at the NBRP B. subtilis, Japan
• 1S126 ( safA::erm), PubMed, available at BGSC
• 1S117 ( safA::spec), [Pubmed| ], available at BGSC
• BKE27840 (ΔsafA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACGTTTTCCCCTCCTATG, downstream forward: _UP4_TGATCGTTCGGAACGATGTA
• BKK27840 (ΔsafA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACGTTTTCCCCTCCTATG, downstream forward: _UP4_TGATCGTTCGGAACGATGTA

Labs working on this gene/protein

• Adriano Henriques, Lisbon, Portugal homepage
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

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Original publications

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