ybfF [2018-05-30 13:09:15]

unknown

locus: BSU02190

pI: 9.67

mw: 35.16 kDa

protein length: 303 aa

gene length: 909 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 238,644 → 239,555

The protein

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|5D159F09BFD98B58A7EDAD37A4DB6468A702D09A|ybfG]-[gene|CEA0A2CE520B8A6E8E9D7C079B954B24137DF686|ybfF]-[gene|3E2A864B904DE4D9F72D2B19257AE81CCED3BE12|ybfE]
  description: [Pubmed|22383849]

  regulation

  • the mRNA is processed between [gene|5D159F09BFD98B58A7EDAD37A4DB6468A702D09A|ybfG] and [gene|CEA0A2CE520B8A6E8E9D7C079B954B24137DF686|ybfF] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

Mutant

• MGNA-B967 (ybfF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1966 NBRP B. subtilis, Japan]
• BKE02190 (Δ[gene|CEA0A2CE520B8A6E8E9D7C079B954B24137DF686|ybfF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTGATAAACCTCCAA, downstream forward: _UP4_TAATTTTGTTTCATCCTTAC
• BKK02190 (Δ[gene|CEA0A2CE520B8A6E8E9D7C079B954B24137DF686|ybfF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTGATAAACCTCCAA, downstream forward: _UP4_TAATTTTGTTTCATCCTTAC