SubtiBank SubtiBank
trmR
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trmR

tRNA wobble uridine modifying methyltransferase
Locus
BSU_27360
Isoelectric point
5.57
Molecular weight
24.92 kDa
Protein length
217 aa Sequence Blast
Gene length
654 bp Sequence Blast
Function
introduction of 5-methoxyuridine modification in tRNA (U34)
Product
tRNA wobble uridine modifying methyltransferase
Essential
no
Synonyms
yrrM

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,795,082 → 2,795,735

The protein

Catalyzed reaction/ biological activity

  • methylation of (5-hydroxyuridine) ho5U containing tRNA to 5-methoxyuridine (mo5U) PubMed
  • 5-hydroxyuridine34 in tRNA + S-adenosyl-L-methionine --> 5-methoxyuridine34 in tRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
  • Protein family

    Cofactors

  • SAM PubMed
  • Structure

  • 5ZW3 PubMed
  • 5ZW4 (bound to tRNA) PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-A853 (yrrM::erm), available at the NBRP B. subtilis, Japan
  • BKE27360 (ΔtrmR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTACAAACAGCCTCCCGT, downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC
  • BKK27360 (ΔtrmR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTACAAACAGCCTCCCGT, downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC
  • References

    Research papers

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