aapA

amino acid permease

Locus: BSU_27090

Isoelectric point: 9.68

Molecular weight: 50.49 kDa

Protein length: 462 aa

Gene length: 1389 bp

Function: amino acid uptake

Product: amino acid permease

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Amino acid transporters → APC superfamily

Gene

Coordinates: 2,766,558 → 2,767,946

The protein

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

Paralogous protein(s)

• GabP, YtnA, HutM, YvbW, YbxG, RocC, RocE, YbgF, YdgF

Structure

• 3NCY (from Salmonella typhimurium, 21% identity) PubMed

Expression and Regulation

Operons

• Genes: aapA
  Description: PubMed

Biological materials

Mutant

• GP2377 ΔaapA::tet, available in Jörg Stülke's lab PubMed
• MGNA-A230 (aapA::erm), available at the NBRP B. subtilis, Japan
• BKE27090 (ΔaapA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTATTTTCCTCCAGT, downstream forward: _UP4_TAAAAAAAAGACAAGGGATA
• BKK27090 (ΔaapA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTATTTTCCTCCAGT, downstream forward: _UP4_TAAAAAAAAGACAAGGGATA

Expression vector

• pGP2279: expression of aapA by pBQ200 in B. subtilis, available in Jörg Stülke's lab

LacZ fusion

• pGP2273 (in pAC5) (GP2960), available in Jörg Stülke's lab

References

Research papers

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