yacF [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

putative tRNA-dihydrouridine synthase B

locus: BSU00810

pI: 7.00

mw: 36.00 kDa

function: tRNA maturation

product: putative tRNA-dihydrouridine synthase B

essential: no

synonyms: dusB, dus1

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

[SW|Categories] containing this gene/protein

• [SW|translation]

Gene

Coordinates on the chromosome (coding sequence): 87,634 -> 88,635

The protein

Catalyzed reaction/ biological activity

• a uridine in tRNA NADPH H → a 5,6-dihydrouridine in tRNA NADP

Protein family

• dus family (according to Swiss-Prot)

Paralogous protein(s)

• [SW|YfjN]

[SW|Cofactors]

• FMN or FAD [Pubmed|21635694]

Structure

• [PDB|1VHN] (from ''Thermotoga maritima'', 35% identity, 54% similarity) [Pubmed|15103641]

Expression and Regulation

Operon

• ''[protein|search|pabB]-[protein|search|pabA]-[protein|search|pabC]-[protein|search|sul]-[protein|search|folB]-[protein|search|folK]-[protein|search|yazB]-[protein|search|yacF]-[protein|search|lysS]'' [Pubmed|9084182]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|9084182]

Additional information

• the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

References

• 12884008,9084182,25902496