SubtiBank SubtiBank
speD
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speD

S-adenosylmethionine decarboxylase, required for biofilm formation
Locus
BSU_29010
Isoelectric point
4.77
Molecular weight
13.96 kDa
Protein length
126 aa Sequence Blast
Gene length
381 bp Sequence Blast
Function
spermidine, polyamine biosynthesis
Product
S-adenosylmethionine decarboxylase
Essential
no
E.C.
4.1.1.50
Synonyms
ytcF

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,966,413 → 2,966,793

Phenotypes of a mutant

The protein

Catalyzed reaction/ biological activity

  • H+ + S-adenosyl-L-methionine --> CO2 + S-adenosyl 3-(methylsulfanyl)propylamine (according to UniProt)
  • Protein family

  • prokaryotic AdoMetDC family (single member, according to UniProt)
  • Structure

  • 1VR7 (from Thermotoga maritima, 47% identity, 72% similarity)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CcpN: repression, PubMed, in CcpN regulon
  • Regulation

  • gapB: repressed in the presence of glucose (CcpN) PubMed
  • Additional information

  • 'speD': the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic 'speD' mRNA increases from 1.4 to 36 min) PubMed
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    Genes
    Description

    Regulation

  • gapB: repressed in the presence of glucose (CcpN) PubMed
  • Additional information

  • 'speD': the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic 'speD' mRNA increases from 1.4 to 36 min) PubMed
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    Biological materials

    Mutant

  • MGNA-A122 (ytcF::erm), available at the NBRP B. subtilis, Japan
  • BKE29010 (ΔspeD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGTCATGGACCCCCCTT, downstream forward: _UP4_TAAAGTAAATTGCGAACACA
  • BKK29010 (ΔspeD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGTCATGGACCCCCCTT, downstream forward: _UP4_TAAAGTAAATTGCGAACACA
  • GP2599 (ΔspeD::tet comIQ12L) (in DK1042), available in Jörg Stülke's lab
  • Expression vector

  • pGP2324: for expression in B. subtilis, in pBQ200,available in Jörg Stülke's lab
  • References

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