You are currently viewing an outdated version of
SubtiWiki.
Please use
the newest version!
speD
S-adenosylmethionine decarboxylase, required for biofilm formation
Molecular weight
13.96 kDa
Function
spermidine, polyamine biosynthesis
Product
S-adenosylmethionine decarboxylase
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
2,966,413 → 2,966,793
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
H+ + S-adenosyl-L-methionine --> CO2 + S-adenosyl 3-(methylsulfanyl)propylamine (according to UniProt)Protein family
prokaryotic AdoMetDC family (single member, according to UniProt)Structure
1VR7 (from Thermotoga maritima, 47% identity, 72% similarity) Expression and Regulation
Operons
Sigma factors
Regulatory mechanism
Regulation
Additional information
'speD': the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic 'speD' mRNA increases from 1.4 to 36 min) PubMed view in new tabRegulation
Additional information
'speD': the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic 'speD' mRNA increases from 1.4 to 36 min) PubMed view in new tabBiological materials
Mutant
MGNA-A122 (ytcF::erm), available at the NBRP B. subtilis, JapanBKE29010 (ΔspeD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGTCATGGACCCCCCTT, downstream forward: _UP4_TAAAGTAAATTGCGAACACABKK29010 (ΔspeD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGTCATGGACCCCCCTT, downstream forward: _UP4_TAAAGTAAATTGCGAACACAGP2599 (ΔspeD::tet comIQ12L) (in DK1042), available in Jörg Stülke's lab Expression vector
References
Loading