SubtiBank SubtiBank
ureC
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ureC

urease (alpha subunit)
Locus
BSU_36640
Isoelectric point
5.09
Molecular weight
61.01 kDa
Protein length
569 aa Sequence Blast
Gene length
1710 bp Sequence Blast
Function
utilization of urea as alternative nitrogen source
Product
urease (alpha subunit)
Essential
no
E.C.
3.5.1.5
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,766,714 → 3,768,423

The protein

Catalyzed reaction/ biological activity

  • 2 H+ + H2O + urea --> CO2 + 2 NH4+ (according to UniProt)
  • Protein family

    Domains

  • Urease domain (aa 132-569) (according to UniProt)
  • Cofactors

  • nickel
  • Structure

  • 5A6T (from Sporosarcina pasteurii, 65% identity) PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigH: sigma factor, PubMed, in SigH regulon
  • Regulatory mechanism

  • GlnR: repression, PubMed, in GlnR regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • PucR: activation, PubMed, in PucR regulon
  • CodY: repression, PubMed, in CodY regulon
  • Regulation

  • induced by nitrogen limitation (GlnR, TnrA) PubMed
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    Biological materials

    Mutant

  • BKE36640 (ΔureC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCATATTCCTCCCGTGACA, downstream forward: _UP4_TGATAAGACGCGGCCGGAGT
  • BKK36640 (ΔureC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCATATTCCTCCCGTGACA, downstream forward: _UP4_TGATAAGACGCGGCCGGAGT
  • References

    Reviews

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    Original publications

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