rocF

arginase

Locus: BSU_40320

Isoelectric point: 4.93

Molecular weight: 32.00 kDa

Protein length: 296 aa

Gene length: 891 bp

Function: arginine utilization

Product: arginase

Essential: no

E.C.: 3.5.3.1

Synonyms: RocF

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of arginine/ ornithine
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 4,141,711 → 4,142,601

The protein

Catalyzed reaction/ biological activity

• H₂O + L-arginine --> L-ornithine + urea (according to UniProt)

Protein family

• arginase family (with SpeB and HutG, according to UniProt)

Modification

• phosphorylated on Ser-68 PubMed

Structure

• 6NFP
• 6DKT

Expression and Regulation

Operons

• Genes: rocD-rocE-rocF
  Description: PubMed

  Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

  Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon
  • AhrC: activation, PubMed, in AhrC regulon
  • CodY: repression, PubMed, in CodY regulon
  • RocR: activation, PubMed, in RocR regulon

  Regulation

  • induced by arginine (RocR, AhrC) PubMed

  Additional information

  • expression of the rocD-rocE-rocF operon is increased upon depletion of nusA (resulting from increased ahrC expression) Reference

Biological materials

Mutant

• GP655, aphA3, available in the Stülke lab
• BKE40320 (ΔrocF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGTGATTCCACCTCAA, downstream forward: _UP4_TAATAAGAAAACCCCCGCAC
• BKK40320 (ΔrocF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGTGATTCCACCTCAA, downstream forward: _UP4_TAATAAGAAAACCCCCGCAC

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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