ndhF [2019-06-17 12:42:05]

NADH dehydrogenase (subunit 5)

locus: BSU01830

pI: 10.15

mw: 55.30 kDa

protein length: 505 aa

gene length: 1518 bp

function: respiration

product: NADH dehydrogenase (subunit 5)

essential: no

synonyms: ybxE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.4|Respiration/ other]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 205,409 → 206,926

The protein

Catalyzed reaction/ biological activity

• NADH quinone = NAD quinol (according to Swiss-Prot)

Protein family

• complex I subunit 5 family (according to Swiss-Prot)

[SW|Cofactors]

• FMN or FAD [Pubmed|21635694]

[SW|Localization]

• cell membrane (according to UniProt), membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]-[gene|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|ybcC]-[gene|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|ybcF]-[gene|EBE0360D89E50EEBB84CC853C6E4B4053DBF8925|ybcH]-[gene|6E063903E9E3B770CAACA9DF98DFA540FAE6FD3D|ybcI]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE01830 (Δ[gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAATTCTCCCTTTT, downstream forward: _UP4_ATTTCATAAGGAGCTAATCT
• BKK01830 (Δ[gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAATTCTCCCTTTT, downstream forward: _UP4_ATTTCATAAGGAGCTAATCT

References

• 18763711