thiF

hydroxyethylthiazole phosphate biosynthesis

locus: BSU_11700

pI: 5.62

mw: 36.24 kDa

protein length: 336 aa

gene length: 1011 bp

function: biosynthesis of thiamine

product: unknown

essential: no

synonyms: yjbU

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]

Gene

Coordinates: 1,245,808 → 1,246,818

The protein

Catalyzed reaction/ biological activity

• [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + ATP + H⁺ --> [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + diphosphate (according to UniProt)

Protein family

• HesA/MoeB/ThiF family (with [protein|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|MoeB] and [protein|37DA3A036937660AA750065B46B581D911D73D00|YrvM], according to UniProt)

Paralogous protein(s)

• [protein|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|MoeB]

Structure

• [PDB|1ZFN] (from ''E. coli'', 35% identity, 51% similarity) [Pubmed|15896804]

Expression and Regulation

Operons

• genes: [gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]
  description: [Pubmed|1898926]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

  regulation

  • repressed by thiamine ([SW|Thi-box]) [Pubmed|16356850]
  • the [SW|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B166 (yjbU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1165 NBRP B. subtilis, Japan]
• BKE11700 (Δ[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCCTGCCTTGAATACCTGC, downstream forward: _UP4_GGGTAATAAAAGGAGTGTAG
• BKK11700 (Δ[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCCTGCCTTGAATACCTGC, downstream forward: _UP4_GGGTAATAAAAGGAGTGTAG

References

Reviews

• 19348578,10382260

Original publications

• 14567704,15896804,1898926,16356850