thiF

hydroxyethylthiazole phosphate biosynthesis

Locus: BSU_11700

Isoelectric point: 5.62

Molecular weight: 36.24 kDa

Protein length: 336 aa

Gene length: 1011 bp

Function: biosynthesis of thiamine

Product: unknown

Essential: no

Synonyms: yjbU

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of thiamine

Gene

Coordinates: 1,245,808 → 1,246,818

The protein

Catalyzed reaction/ biological activity

• [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + ATP + H⁺ --> [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + diphosphate (according to UniProt)

Protein family

• HesA/MoeB/ThiF family (with MoeB and YrvM, according to UniProt)

Paralogous protein(s)

• MoeB

Structure

• 1ZFN (from E. coli, 35% identity, 51% similarity) PubMed

Expression and Regulation

Operons

• Genes: tenA-tenI-thiO-thiS-thiG-thiF-thiD
  Description: PubMed

  Regulatory mechanism

  • Thi-box: RNA switch, via RNA switch, in Thi-box regulon

  Regulation

  • repressed by thiamine (Thi-box) PubMed
  • the Thi-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• MGNA-B166 (yjbU::erm), available at the NBRP B. subtilis, Japan
• BKE11700 (ΔthiF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTCCTGCCTTGAATACCTGC, downstream forward: _UP4_GGGTAATAAAAGGAGTGTAG
• BKK11700 (ΔthiF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTCCTGCCTTGAATACCTGC, downstream forward: _UP4_GGGTAATAAAAGGAGTGTAG

References

Reviews

Loading

Original publications

Loading