gidB [2019-01-30 12:55:29]

7-methylguanosine methyltransferase specific for the 16S rRNA, glucose-inhibited division protein

Locus: BSU41000

Isoelectric point: 6.60

Molecular weight: 26.80 kDa

Protein length: 239 aa

Gene length: 720 bp

Function: rRNA modification

Product: 7-methylguanosine methyltransferase specific for the 16S rRNA

Essential: no

Synonyms: rsmG

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → rRNA modification and maturation

Gene

Coordinates: 4,208,870 → 4,209,589

Phenotypes of a mutant

• low-level resistance to streptomycin, increased level of streptomycin-resistant rpsL mutants (200 times higher than in the wild type) PubMed

The protein

Catalyzed reaction/ biological activity

• methylates the N7 position of nucleotide G535 in 16S rRNA PubMed

Protein family

• RNA methyltransferase rsmG family (according to Swiss-Prot)

Structure

• 1XDZ

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: thdF-gidA-gidB-noc
  Description: PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DnaA: repression, PubMed, in DnaA regulon

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• MGNA-B875 (gidB::erm), available at the NBRP B. subtilis, Japan
• BKE41000 (ΔgidB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAATATTCATGCCGTCAT, downstream forward: _UP4_TAATTTAGACAGCCTGATAT
• BKK41000 (ΔgidB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAATATTCATGCCGTCAT, downstream forward: _UP4_TAATTTAGACAGCCTGATAT

References

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