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gidB
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gidB

7-methylguanosine methyltransferase specific for the 16S rRNA, glucose-inhibited division protein
Locus
BSU_41000
Isoelectric point
6.60
Molecular weight
26.80 kDa
Protein length
239 aa Sequence Blast
Gene length
720 bp Sequence Blast
Function
rRNA modification
Product
7-methylguanosine methyltransferase specific for the 16S rRNA
Essential
no
Synonyms
rsmG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → rRNA modification and maturation

    • Gene

    Coordinates
    4,208,870 → 4,209,589

    Phenotypes of a mutant

  • low-level resistance to streptomycin, increased level of streptomycin-resistant rpsL mutants (200 times higher than in the wild type) PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • methylates the N7 position of nucleotide G535 in 16S rRNA PubMed

    • Protein family

  • Methyltransferase superfamily (according to UniProt)

    • Structure

  • 1XDZ

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    thdF-gidA-gidB-noc
    Description
    PubMed

    Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DnaA: repression, PubMed, in DnaA regulon

    • Regulation

  • constitutively expressed PubMed
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    Biological materials

    Mutant

  • MGNA-B875 (gidB::erm), available at the NBRP B. subtilis, Japan
  • BKE41000 (ΔgidB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAATATTCATGCCGTCAT, downstream forward: _UP4_TAATTTAGACAGCCTGATAT
  • BKK41000 (ΔgidB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAATATTCATGCCGTCAT, downstream forward: _UP4_TAATTTAGACAGCCTGATAT

    • References

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