SubtiBank SubtiBank
ywbI [2019-09-03 09:27:47]
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ywbI [2019-09-03 09:27:47]

transcriptional regulator ([SW|LysR family]), activator of the [gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG] operon
locus
BSU_38310
pI
5.93
mw
34.52 kDa
protein length
301 aa Sequence Blast
gene length
906 bp Sequence Blast
function
control of expression of the [gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG] operon
product
transcriptional regulator ([SW|LysR family])
essential
no
synonyms
ipa-24d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]

    • Gene

    Coordinates
    3,932,198 → 3,933,103

    The protein

    Protein family

  • [SW|LysR family] (according to UniProt)

    • Effectors of protein activity

  • acetate activates [protein|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|YwbI] to bind DNA [Pubmed|26060272]

    • Structure

  • [PDB|3FZV] (from Pseudomonas aeruginosa, 28% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]-[gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]-[gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]
    description
    [Pubmed|9139923]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9139923], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • MGNA-B675 (ywbI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1674 NBRP B. subtilis, Japan]
  • BKE38310 (Δ[gene|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGCTTCACCCTTTCTA, downstream forward: _UP4_AAAGATAGTAAAGGATGATG
  • BKK38310 (Δ[gene|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGCTTCACCCTTTCTA, downstream forward: _UP4_AAAGATAGTAAAGGATGATG

    • References

  • 9139923,26060272