ycgG

unknown

locus: BSU_03100

pI: 9.19

mw: 29.04 kDa

protein length: 253 aa

gene length: 762 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 335,329 → 336,090

The protein

Protein family

• DcsA family (with [protein|0B5A1E73710CF6A158925CC51E170BA7BEF3B495|YqcI], according to UniProt)

Paralogous protein(s)

• [protein|0B5A1E73710CF6A158925CC51E170BA7BEF3B495|YqcI]

Expression and Regulation

Operons

• genes: [gene|D948867BAEF91943250714453A474B90AB64E523|ycgE]-[gene|78B3F9580DED32469ACF537F4B40C7DBF0D4D995|ycgF]-[gene|CBEC4D299232FC5FCB57211525404CBD42D5AF68|ycgG]
  description: [Pubmed|22383849]

• genes: [gene|78B3F9580DED32469ACF537F4B40C7DBF0D4D995|ycgF]-[gene|CBEC4D299232FC5FCB57211525404CBD42D5AF68|ycgG]
  description: [Pubmed|22383849]

• genes: [gene|CBEC4D299232FC5FCB57211525404CBD42D5AF68|ycgG]
  description: [Pubmed|12662922]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • subject to negative regulation by [SW|SpoIIID] [Pubmed|15383836]

Biological materials

Mutant

• MGNA-B988 (ycgG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1987 NBRP B. subtilis, Japan]
• GP1851 (Δ[gene|CBEC4D299232FC5FCB57211525404CBD42D5AF68|ycgG]::aphA3)), available in [SW|Jörg Stülke]'s lab [Pubmed|25092907]
• BKE03100 (Δ[gene|CBEC4D299232FC5FCB57211525404CBD42D5AF68|ycgG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATCCACCTCCAGTAT, downstream forward: _UP4_AACATGTTCAACAAAATGAA
• BKK03100 (Δ[gene|CBEC4D299232FC5FCB57211525404CBD42D5AF68|ycgG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATCCACCTCCAGTAT, downstream forward: _UP4_AACATGTTCAACAAAATGAA

Expression vectors

• IPTG inducible expression of Strep-''ycgG'' in ''E. coli'': pGP2578 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

References

• 12662922,15383836,23420519,25092907