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era [2019-10-04 10:08:21]
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era [2019-10-04 10:08:21]

GTP-binding protein, involved in the assembly of the 30S ribosomal subunit
locus
BSU_25290
pI
8.38
mw
33.92 kDa
protein length
301 aa Sequence Blast
gene length
906 bp Sequence Blast
function
ribosome assembly
product
GTP-binding protein
essential
yes
synonyms
bex,yqfH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

    • Gene

    Coordinates
    2,610,041 → 2,610,946

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Binds and hydrolyzes GTP and readily exchanges GDP for GTP

    • Protein family

  • [SW|TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|YsxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|RbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|ThdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|Obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|YqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|YnbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|YphC], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|YyaF]

    • [SW|Domains]

  • Era-type G domain (aa 7-174) (according to UniProt)
  • KH type-2 domain (aa 205-282) (according to UniProt)

    • Effectors of protein activity

  • binds ppGpp [Pubmed|26951678], ppGpp may inhibit activity [Pubmed|26951678]

    • Structure

  • [PDB|3IEV] ([protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|Era] from ''Aquifex aeolicus'', 50% identity/ 77% similarity, complex with the 3' end of [SW|16S rRNA]) [Pubmed|19706445]

    • Expression and Regulation

    Operons

    genes
    [gene|0E9E9615CE056BEA2B65C494D946EB45F42A3064|cdd]-[gene|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era]-[gene|33FBC31E8C24177137B0BDD8B46C1AFFD7F7B038|yqzL]-[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|6330116], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|6330116], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
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    Biological materials

    Expression vectors

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP837, available in [SW|Stülke] lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP845, available in [SW|Stülke] lab

    • labs

  • [SW|Naotake Ogasawara], Nara, Japan

    • References

    Reviews

  • 19575570,21885683

    • Original publications

  • 12427945,6330116,1970644526951678,27382067