mtnU

omega-amidase

locus: BSU_13570

pI: 5.10

mw: 29.25 kDa

protein length: 259 aa

gene length: 780 bp

function: methionine salvage

product: omega-amidase

essential: no

synonyms: ykrU

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

Gene

Coordinates: 1,424,767 → 1,425,546

Phenotypes of a mutant

• reduced growth with 5-methylthioribose as single sulfur source [Pubmed|24837359]

The protein

Catalyzed reaction/ biological activity

• 2-ketoglutaramate + H₂O --→ 2-oxoglutarate + NH₃ [Pubmed|24837359]

Protein family

• carbon-nitrogen hydrolase superfamily (with [protein|BDAA216E832B8EB267CED6456A0045254DD0E97D|YhcX], according to UniProt)

[SW|Domains]

• CN hydrolase (aa 3-238) (according to UniProt)

Structure

• [PDB|3P8K] (from Staphylococcus aureus, 42% identity)

Expression and Regulation

Operons

• genes: [gene|CB12BCCF6CEADC0C64DD918E0BAE4748E358A67B|mtnU]
  description: [Pubmed|11545674]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12022921], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-A784 (ykrU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/784 NBRP B. subtilis, Japan]
• BKE13570 (Δ[gene|CB12BCCF6CEADC0C64DD918E0BAE4748E358A67B|mtnU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTAACACCCCAAA, downstream forward: _UP4_TAAAAAATATATTGACAACT
• BKK13570 (Δ[gene|CB12BCCF6CEADC0C64DD918E0BAE4748E358A67B|mtnU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTAACACCCCAAA, downstream forward: _UP4_TAAAAAATATATTGACAACT

References

• 15102328,12022921,11545674,24837359