pksE

involved in polyketide synthesis

Locus: BSU_17120

Isoelectric point: 7.38

Molecular weight: 85.61 kDa

Protein length: 767 aa

Gene length: 2304 bp

Function: polyketide synthesis

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds

Gene

Coordinates: 1,786,104 → 1,788,407

The protein

Catalyzed reaction/ biological activity

• holo-[ACP] + malonyl-CoA --> CoA + malonyl-[ACP] (according to UniProt)

Protein family

• fabD family (with PksC and FabD, according to UniProt)

Structure

• 5DZ7 (N-terminal domain, aa 1 ... 288)
• 4CW5 (C-terminal domain, from B. amyloliquefaciens, corresponds to aa 315 ... 753, 59% identity) PubMed

Expression and Regulation

Operons

• Genes: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • CodY: activation, PubMed, in CodY regulon

  Regulation

  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed

  Additional information

  • this is a very large operon comprising about 75 kb

• Genes: pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
  Description: PubMed

Biological materials

Mutant

• BKE17120 (ΔpksE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCCCTGGAAAGACATATG, downstream forward: _UP4_TGAATATTATGAAAATATAC
• BKK17120 (ΔpksE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGCCCTGGAAAGACATATG, downstream forward: _UP4_TGAATATTATGAAAATATAC

References

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