pgcM

beta-phosphoglucomutase/ glucose-1-phosphate phosphodismutase

locus: BSU_34550

pI: 4.76

mw: 24.95 kDa

protein length: 226 aa

gene length: 681 bp

function: starch and maltodextrin utilization

product: beta-phosphoglucomutase(EC 5.4.2.6 and EC 2.7.1.41)

essential: no

ec: 5.4.2.6

synonyms: yvdM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.23|Utilization of starch/ maltodextrin]

Gene

Coordinates: 3,546,873 → 3,547,553

The protein

Catalyzed reaction/ biological activity

• β-D-glucose 1-phosphate --> β-D-glucose 6-phosphate (according to UniProt)

Protein family

• [SW|HAD superfamily] (according to UniProt)
• CbbY/CbbZ/Gph/YieH family (with [protein|3DB9048B54C47E262997A2A5A0556AE607F93EA9|YhcW], according to UniProt)

Paralogous protein(s)

• [protein|345EC248C0ADF48B0C25B175241AAE982E0E9B78|YhdA]:
• [protein|3DB9048B54C47E262997A2A5A0556AE607F93EA9|YhcW]:

Structure

• [PDB|3NAS]

Expression and Regulation

Operons

• genes: [gene|EA1BEC9FCFD2A32142A8A8E6D7362DB37E02052A|yvdE]-[gene|A577441DE4ABAF624D507DB80FBED2A652BCF385|yvdF]-[gene|5771E1976D986638B13ADDE97EDE32709E355923|mdxE]-[gene|EA2DD9892C1128ADCD6C8BC61248EBE45339E6D6|mdxF]-[gene|D72CFFE8ED3EC0280AE92C05A136D66ED1F62251|mdxG]-[gene|68C6FD5D4D9491EE15A5015447959A45C36EA702|yvdJ]-[gene|5F615BB886EC4F7000AAD42258DB8E47616552DE|yvdK]-[gene|6E5AB4620F9A896C32CD77AF314C67035814AE0A|malL]-[gene|CAFAE305B1771DD89E81F75A6713161393BE99A0|pgcM]
  description: [Pubmed|16707683]

  regulation

  • induced in the presence of maltose [Pubmed|9573215]

Biological materials

Mutant

• MGNA-B622 (yvdM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1621 NBRP B. subtilis, Japan]
• BKE34550 (Δ[gene|CAFAE305B1771DD89E81F75A6713161393BE99A0|pgcM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTAAATCAAAAATGACCG, downstream forward: _UP4_TGAAATGAAAAAAACCTGCA
• BKK34550 (Δ[gene|CAFAE305B1771DD89E81F75A6713161393BE99A0|pgcM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTAAATCAAAAATGACCG, downstream forward: _UP4_TGAAATGAAAAAAACCTGCA

References

• 11081794,16707683