yngH

methylcrotonoyl-CoA carboxylase

Locus: BSU_18240

Isoelectric point: 7.31

Molecular weight: 48.92 kDa

Protein length: 444 aa

Gene length: 1335 bp

Function: mother cell metabolism, leucine utilization

Product: methylcrotonoyl-CoA carboxylase, subunit

Essential: no

E.C.: 6.4.1.4

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 1,953,181 → 1,954,515

The protein

Catalyzed reaction/ biological activity

• ATP + hydrogencarbonate + N⁶-biotinyl-L-lysyl-[protein] --> ADP + H⁺ + N⁶-carboxybiotinyl-L-lysyl-[protein] + phosphate (according to UniProt)
• acetyl-CoA + ATP + hydrogencarbonate --> ADP + H⁺ + malonyl-CoA + phosphate (according to UniProt)

Domains

• ATP-grasp domain (aa 120-317) (according to UniProt)
• Biotin carboxylation domain (aa 1-444) (according to UniProt)

Cofactors

• biotin

Structure

• 1ULZ (biotin carboxylase subunit of pyruvate carboxylase from Aquifex aeolicus, 52% identity) PubMed

Expression and Regulation

Operons

• Genes: yngJ-yngI-yngH-yngHB-yngG-yngF-yngE
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulation

  • expressed during sporulation in the mother cell (SigE) PubMed

Biological materials

Mutant

• MGNA-B086 (yngH::erm), available at the NBRP B. subtilis, Japan
• BKE18240 (ΔyngH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TACTTTTGTAAACATGATTT, downstream forward: _UP4_CACCTATAAAGGAGGCAGTT
• BKK18240 (ΔyngH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TACTTTTGTAAACATGATTT, downstream forward: _UP4_CACCTATAAAGGAGGCAGTT

References

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