yngH

methylcrotonoyl-CoA carboxylase

locus: BSU_18240

pI: 7.31

mw: 48.92 kDa

protein length: 444 aa

gene length: 1335 bp

function: mother cell metabolism, leucine utilization

product: methylcrotonoyl-CoA carboxylase, subunit

essential: no

ec: 6.4.1.4

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 1,953,181 → 1,954,515

The protein

Catalyzed reaction/ biological activity

• ATP + hydrogencarbonate + N⁶-biotinyl-L-lysyl-[protein] --> ADP + H⁺ + N⁶-carboxybiotinyl-L-lysyl-[protein] + phosphate (according to UniProt)
• acetyl-CoA + ATP + hydrogencarbonate --> ADP + H⁺ + malonyl-CoA + phosphate (according to UniProt)

[SW|Domains]

• [SW|ATP-grasp domain] (aa 120-317) (according to UniProt)
• Biotin carboxylation domain (aa 1-444) (according to UniProt)

[SW|Cofactors]

• biotin

Structure

• [PDB|1ULZ] (biotin carboxylase subunit of pyruvate carboxylase from Aquifex aeolicus, 52% identity) [Pubmed|14993673]

Expression and Regulation

Operons

• genes: [gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]
  description: [Pubmed|12662922]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]

Biological materials

Mutant

• MGNA-B086 (yngH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1085 NBRP B. subtilis, Japan]
• BKE18240 (Δ[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACTTTTGTAAACATGATTT, downstream forward: _UP4_CACCTATAAAGGAGGCAGTT
• BKK18240 (Δ[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACTTTTGTAAACATGATTT, downstream forward: _UP4_CACCTATAAAGGAGGCAGTT

References

• 15699190,12662922,19935659,14993673