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levE
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levE

fructose-specific permease of the phosphotransferase system, EIIB of the PTS, Trigger enzyme, control of LevR activity
Locus
BSU_27060
Isoelectric point
9.44
Molecular weight
17.94 kDa
Protein length
162 aa Sequence Blast
Gene length
489 bp Sequence Blast
Function
fructose uptake and phosphorylation, control of LevR activity
Product
fructose-specific PTS, EII component
Essential
no
E.C.
2.7.1.69
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of other polymeric carbohydrates
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of fructose
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of PRD-type regulators
  • Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue

    • Gene

    Coordinates
    2,761,907 → 2,762,395

    The protein

    Catalyzed reaction/ biological activity

  • D-fructose + Nπ-phospho-L-histidyl-[protein] --> D-fructose 1-phosphate + L-histidyl-[protein] (according to UniProt)

    • Protein family

  • PTS permease, mannose family PubMed

    • Domains

  • PTS EIIB domain type-4 (aa 1-163) (according to UniProt)

    • Structure

  • 1BLE

    • Localization

  • cytoplasm PubMed

    • Expression and Regulation

    Operons

    Genes
    levD-levE-levF-levG-sacC
    Description
    PubMed

    Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • LevR: activation, PubMed, in LevR regulon

    • Regulation

  • induced in the presence of fructose (LevR) PubMed
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    Biological materials

    Mutant

  • BKE27060 (ΔlevE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATAATTCATCCTCTCT, downstream forward: _UP4_ACAAAATAATCAAGGGGATG
  • BKK27060 (ΔlevE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATAATTCATCCTCTCT, downstream forward: _UP4_ACAAAATAATCAAGGGGATG

    • References

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