ypnP

putative damage inducible, Na+ driven multidrug efflux pump

locus: BSU_21710

pI: 10.05

mw: 48.31 kDa

protein length: 445 aa

gene length: 1338 bp

function: unknown

product: putative damage inducible, Na+ driven multidrug efflux pump

essential: no

synonyms: dinF

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.15|Multidrug exporters/ based on homology]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,288,669 → 2,290,006

The protein

Protein family

• [SW|MOP exporter family]
• [SW|multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family] (according to UniProt)

Structure

• [PDB|3VVO] (MATE multidrug exporter from Pyrococcus furiosus, 25% identity) [pubmed|23535598]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|CA85B14720E86D2E609388D561B56ADA880B2190|ypnP]
  description: (according to [http://dbtbs.hgc.jp/COG/prom/ypnP.html DBTBS]) null

Biological materials

Mutant

• MGNA-A890 (ypnP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/890 NBRP B. subtilis, Japan]
• BKE21710 (Δ[gene|CA85B14720E86D2E609388D561B56ADA880B2190|ypnP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCTCCTTGTGTTC, downstream forward: _UP4_TAAGTGAAGCACTGCGAAAA
• BKK21710 (Δ[gene|CA85B14720E86D2E609388D561B56ADA880B2190|ypnP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCTCCTTGTGTTC, downstream forward: _UP4_TAAGTGAAGCACTGCGAAAA

References

Research papers

• 23535598