thrZ [2017-8-11 16:0:3]

threonyl-tRNA synthetase (minor)

locus: BSU37560

pI: 5.75

mw: 73.20 kDa

protein length: 638 aa

gene length: 1914 bp

function: translation

product: threonyl-tRNA synthetase (minor)

essential: no

ec: 6.1.1.3

synonyms: thrS2

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]

Gene

Coordinates: 3,854,256 → 3,856,172

The protein

Catalyzed reaction/ biological activity

• ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr) (according to Swiss-Prot)

Protein family

• class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|ThrS], one of the two proteins has to be present for viability [Pubmed|17114254]

Structure

• [PDB|1TJE] (from ''Escherichia coli'', 43% identity, 65% similarity) [Pubmed|15525511]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|CA74CB721842927F67FBC3B5C27D2EE46B30CE1C|thrZ]-[gene|4A3CA47533B217F595E7B600923DA31228B94DA8|ywhA]
  description: [Pubmed|1379177]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1379177], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: anti-termination, in [regulon|T-box|T-box]

  regulation

  • induced by threonine limitation ([SW|T-box]) [Pubmed|19258532]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

References

• 19258532,8288542,1379177,17114254,21815947