mcsA [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

modulator of CtsR-dependent repression, McsA activates kinase activity of McsB

locus: BSU00840

pI: 6.62

mw: 20.00 kDa

function: control of CtsR activity

product: activator of McsB kinase activity

essential: no

synonyms: yacH

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

[SW|Categories] containing this gene/protein

• [SW|transcription factors and their control], [SW|sporulation proteins], [SW|general stress proteins (controlled by SigB)], [SW|heat shock proteins], [SW|phosphoproteins]

This gene is a member of the following [SW|regulons]

• [SW|CtsR regulon], [SW|SigB regulon], [SW|SigF regulon]

Gene

Coordinates on the chromosome (coding sequence): 101,927 -> 102,484

The protein

Modification

• phosphorylated on Arg-169 [Pubmed|22517742]

[SW|Interactions]

• [protein|search|McsA]-[protein|search|McsB]-[protein|search|CtsR], [protein|search|McsA]-[protein|search|McsB]

Expression and Regulation

Operon

• ''[protein|search|ctsR]-[protein|search|mcsA]-[protein|search|mcsB]-[protein|search|clpC]-[protein|search|radA]-[protein|search|disA]'' [Pubmed|8793870]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|8793870], [protein|search|SigB] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2], [protein|search|SigF] [Pubmed|16497325]

Regulation

• expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325]
• induced by stress ([protein|search|SigB]) [Pubmed|11544224]
• induced by heat ([protein|search|CtsR]) [Pubmed|9987115]

Regulatory mechanism

• [protein|search|CtsR]: transcription repression [Pubmed|9987115]

Biological materials

Mutant

• ''mcsA::aphA3'' available from the Gerth lab

Expression vector

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

Antibody

• available in Gerth lab

References

• 12923084,8793870,11179229,9987115,16497325,19226326,9987115,11544224,22517742