aroE

3-phosphoshikimate 1-carboxyvinyltransferase

locus: BSU_22600

pI: 6.34

mw: 45.08 kDa

protein length: 428 aa

gene length: 1287 bp

function: biosynthesis of aromatic amino acids

product: 3-phosphoshikimate 1-carboxyvinyltransferase

essential: yes

ec: 2.5.1.19

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.13|Biosynthesis/ acquisition of aromatic amino acids]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,367,954 → 2,369,240

Phenotypes of a mutant

• essential [Pubmed|28189581,30666812]

The protein

Catalyzed reaction/ biological activity

• 3-phosphoshikimate + phosphoenolpyruvate --> 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate (according to UniProt)

Protein family

• EPSP synthase family (with [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|MurAA] and [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|MurAB], according to UniProt)

Effectors of protein activity

• inhibited by glyphosate [pubmed|30666812]

Structure

• [PDB|3RMT] (from ''B. halodurans'', 68% identity, 87% similarity)

Expression and Regulation

Operons

• genes: [gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]-[gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]-[gene|DEB8358CE34F80560BE91F036659EF2C67A19623|tyrA]-[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]
  description: [Pubmed|21815947]

  regulatory mechanism

  • [protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]: termination/antitermination, [pubmed|1551827], in [regulon|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB regulon]

  regulation

  • not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]) [Pubmed|1551827]
  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

• genes: [gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]-[gene|DEB8358CE34F80560BE91F036659EF2C67A19623|tyrA]-[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]
  description: [Pubmed|21815947]

  additional information

  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• BKE22600 (Δ[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22600 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CTTATCTCGTTTCATTTTTT, downstream forward: _UP4_TGAAGTTTTACTTCAGGATT

Expression vector

• pIGEM_2: constitutive overexpression of [gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE] by pBQ200 in B. subtilis, available in [SW|Jörg Stülke's ] and [SW|Fabian Commichau's] labs

Expression vectors

• for expression in ''B. subtilis'', in [SW|pBQ200]: piGEM2, available in [SW|Fabian Commichau]'s lab

labs

• [SW|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]

References

Reviews

• 12966138

Original publications

• 3924737,3106153,6436812,1551827,8419914,21815947,28189581,30666812, 3111378, 7744055